SEROLOGICAL AND MOLECULAR CHARACTERISTICS OF TOMATO MOSAIC TOBAMOVIRUS COAT PROTEIN

Monoclonal Antibodies to Identify Tomato Mosaic Tobamovirus (tomv)

Monoclonal antibodies were obtained against Tomato mosaic tobamovirus (ToMV) isolated in Brazil. One antibody (8G7G2) isotyped as IgG2b (κ light chain) showed strong specificity and very low cross reaction with the Tobacco mosaic virus (TMV). It can be used in identification of tomato mosaic virus (ToMV).

Purification, Partial Characterization, and Serological Comparison of Soybean Mosaic Virus and its Coat Protein

SOONG, M. M., and G. M. MILBRATH. 1980. Purification, partial characterization, and serological comparison of soybean mosaic virus and its coat protein. Phytopathology 70:388-391. An Illinois isolate of soybean mosaic virus (SMV-IL) was purified from frequent length of 675-750 nm. Infectious SMV RNA was separated from cultivar Kanrich soybean leaves, 16-18 days after inoculation by protein bydi...

Characterization of Coat Protein Gene of Cucumber Mosaic Virus Isolates in Iran

Background: Cucumber mosaic virus (CMV) from the Bromoviridae family, is one of the most widespread plant viruses in the world. Objectives: In the present study tomato fields in Guilan, Isfahan, Khorasan Razavi, Khuzestan and Tehran provinces were surveyed to determine the presence of CMV subgroups during 2011-2012. Materials and Methods: Out of 305 symptomatic leaf samples ...

serological methods to confirm expression of coat protein gene from an iranian isolate of cucumber mosaic virus in escherichia coli

conclusions this is the first report of expression of cmv cp gene in iran, which is important for the preparation of anti-cmv antibody and paving the way for the use of the virus coat protein as a nanomaterial. results the identity of the expressed protein was confirmed by immunoassays such as western blot, dot-immunobinding assay (diba) and enzyme-linked immunosorbent assay (elisa) by the use ...

Partial Epitope Mapping of Alfalfa mosaic Virus and the Effect of Coat Protein Gene Mutation on Aphid Transmission